1. Laura Palmer
She gave the show its central mystery, and its zeitgeist-conquering catch phrase: Who killed Laura Palmer? But even though her death is literally what made the story possible, it's her life that made it matter. Unlike the macabre MacGuffins of so many post-Peaks dead-girl mysteries, Laura was not a beautiful cipher, existing solely to inspire the male detectives investigating her murder. She was a vibrant, complicated character in her own right, the person who best embodied the small-town-secrets theme, and who paid the highest price for those secrets. Her life, and the suffering that ended it, were always foregrounded. And our glimpses of her in the series – a videotape, an audio recording, a diary entry, a visitation from Another Place – were all merely a prelude to her starring role in the prequel film Fire Walk With Me, featuring actor Sheryl Lee's tear-down-the-sky performance of a character coming to grips with the most profound cruelty imaginable. "She's dead, wrapped in plastic"? Yes. But she'll live forever.