29. Alyx Vance
Alyx Vance was a breath of fresh air in 2004, a woman in a video game who dressed sensibly and whose introduction has her performing the rescue. She's still an attractive lady with an inexplicable crush on our hero Gordon Freeman, but Alyx was a step forward for portrayals of women. Half-Life 2 was also a step forward in terms of facial animation, and Alyx benefited from this most. Though Gordon is the silent one, Alyx communicates as easily with her expressive face as with her words.
Iconic moment: Alyx's face fills the screen as she looks down at Gordon, who has just been knocked to the ground. Unconcerned, she jokes, "Doctor Freeman, I presume?" with a goofy grin, flicking to a worried frown an instant later as alarms sound in the distance.